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SRX4451764: Metagenome sequences of anammox biomass
1 ILLUMINA (Illumina HiSeq 2500) run: 3.3M spots, 2G bases, 1.1Gb downloads

Design: NEB Next Ultra II DNA library
Submitted by: KAUST
Study: Application of an enrichment culture of the marine anammox bacterium Ca . Scalindua for nitrogen removal under moderate salinity and in the presence of organic carbon
show Abstracthide Abstract
Seawater can be directly used for toilet flushing in coastal areas to reduce our dependence on desalination and freshwater resources. The presence of high salt content in the generated wastewater from seawater toilet flushing could limit the performance of conventional biological nitrogen removal processes. Anaerobic ammonium oxidation (anammox) process is regarded as one of the most energy-efficient processes for nitrogen removal from N-rich waste streams. In this study, we demonstrated the application of a novel marine anammox bacterium (Candidatus Scalindua sp. AMX11) in a membrane bioreactor (MBR) to treat moderate-saline (1.2% salinity) and N-rich organic (2 mM acetate) solution, prepared using real seawater. The MBR showed stable performance with nitrogen removal rate of 0.3 kg N m3 d1 at >90% N-removal efficiency. Furthermore, results of 15N stable isotope experiments revealed that anammox bacteria was mainly responsible for respiratory ammonification through NO3 reduction to NH4+ via NO2, and the by-products of respiratory ammonification were used as substrates by anammox bacteria. The dominant role of anammox bacteria in nitrogen removal under saline and organic conditions was further confirmed by genome-centric combined metagenomics and meta-transcriptomic approach. Taken together, these results highlight the potential application of marine anammox bacteria for treating saline wastewater generated from seawater toilet flushing practices.
Sample: Metagenome or environmental sample from Marine ANAMMOX Bioreactor
SAMN09700846 • SRS3590464 • All experiments • All runs
Library:
Name: MQ180126-73
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: METAGENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 3.3M spots, 2G bases, 1.1Gb
Run# of Spots# of BasesSizePublished
SRR75859053,338,0342G1.1Gb2019-08-22

ID:
6034668

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